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. 2018 Jul 9;8:254. doi: 10.3389/fonc.2018.00254

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Copyright © 2018 Gibellini, Losi, De Biasi, Nasi, Lo Tartaro, Pecorini, Patergnani, Pinton, De Gaetano, Carnevale, Pisciotta, Mariani, Roncucci, Iannone, Cossarizza and Pinti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LonP1 modulates glycolytic activity in colon cancer cells. (A) Representative histograms showing glucose transporter (GLUT)-1 expression in SW480-pMSCV, SW480-pLonP1, SW620-pMSCV, and SW620-pLonP1 cells. Quantitative analysis is reported on the right and shows the median fluorescence intensity of plasma membrane GLUT-1 levels, as revealed by flow cytometry. (B) Representative Western blot analysis and relative protein levels of phosphorylated lactate dehydrogenase A and glucose 6-phosphate dehydrogenase in SW480 and SW620 cells overexpressing LonP1. β-actin was used as loading control. Densitometries are reported in histograms, and data are reported as mean ± SD (n = 3). *P < 0.05. (C) Representative traces and quantitative analysis of extracellular acidification rate in indicated cells. Subsequent injections of glucose (Glu), oligomycin (Oligo), and 2-deoxy-glucose were performed as indicated.