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. 2018 May 19;5:647–653. doi: 10.1016/j.toxrep.2018.05.011

Fig. 2.

Fig. 2

Effects of KPA on expression of iNOS in LPS-stimulated J774 A.1 cells. Cells were treated with KPA (25-100 μM) for 1 h prior to LPS (1 μg/ml) stimulation for 24 h. (A) The expression of iNOS at the mRNA was determined by RT-PCR and the representation of iNOS mRNA was analyzed by densitometry protocol. (B) The expression of iNOS at the protein levels was determined by western blotting and the representation of iNOS protein was analyzed by densitometry protocol. Values represent the mean ± SD of three independent experiments and statistical significant values are indicated (*p < 0.05, compared with LPS-stimulated group).