Table 4. Rat IP1, GK Rat oGTT, BSEP, and Off-Target Activity Comparison of Key Compounds.

Cmpd	rat IP1a (EC50 nm ± SD)	GK rat oGTT MEDb (pl concc @ 3 h, dose)	Off-target Hitsd #	BSEPe (IC50 μM ± SEM)
14	1.2 ± 0.35	3.0 μm @ 10 mg/kg	9	1.4 ± 0.1
16	2.3 ± 0.40	13.9 μm @ 30 mg/kg	3	0.3 ± 0.04
AP5	0.49 ± 0.28	4.9 μm @ 10 mg/kg	4	3.5 ± 0.2
29	5.2 ± 1.6	32 μm @ 30 mg/kg	1	3.2 ± 0.4

^aMean of at least two runs, % activation >370%.²²

^bMED = maximum efficacious dose.

^cpl conc = plasma concentration.

^d>50% inhibition @ 10 μM; see Supporting Information for specific receptors.

^eMean of three measurements.