Figure 4.

Effect of pathogen-associated molecular patterns (PAMPs) on the survival of adult zebrafish after a Spring Viremia Carp Virus (SVCV) challenge. (A) Kaplan–Meier survival curves after an SVCV i.p. challenge. Fish were stimulated with PAMPs or inoculated with phosphate-buffered saline (PBS), and 35 days after a single-dose administration, they were i.p. challenged with SVCV. No significant differences were observed among the different groups, although the PAMP-treated zebrafish showed a lower survival rate. (B) Kaplan–Meier survival curves after SVCV i.p. challenge. Fish were previously inoculated with β-glucans or PBS and i.p. challenged with SVCV 7 days after a single-dose administration. A slight but significant increase in the survival rate was observed in fish that were previously treated with β-glucans. Significant differences are represented by asterisks (*p < 0.05).