Table 1.
Top 25 most up- and downregulated genes in β-glucan-treated zebrafish compared to unstimulated fish in the absence (A) or presence (B) of Spring Viremia Carp Virus (SVCV) infection.
| (A) Top 25-DEGs glucans vs. phosphate-buffered saline (PBS) | |
| Gene name | FC |
| Upregulated genes | |
| F-box protein 36a | 4.1 |
| G protein-coupled receptor 35, tandem duplicate 1 | 4.0 |
| Crystallin, gamma M2c | 3.8 |
| Golgin subfamily A member 6-like protein 22 | 3.3 |
| Troponin I type 1b | 3.2 |
| Fibronectin type III domain-containing protein 4-like | 3.1 |
| Tumor necrosis factor receptor superfamily, member 9b | 3.0 |
| Phospholipase C-like 1 | 3.0 |
| Adhesion G protein-coupled receptor F8 | 2.8 |
| Relaxin/insulin-like family peptide receptor 3.3b | 2.8 |
| ERBB receptor feedback inhibitor 1 | 2.7 |
| Inner membrane protein, mitochondrial (mitofilin) | 2.6 |
| Heterogeneous nuclear ribonucleoprotein A3 | 2.4 |
| PDZ domain containing 3b | 2.4 |
| Testis-enhanced gene transcript (BAX inhibitor 1) | 2.4 |
| Cerebellin 9 | 2.4 |
| Sema domain, immunoglobulin domain (Ig), short basic domain, secreted (semaphorin) 3bl | 2.3 |
| Protein phosphatase 6, regulatory subunit 2 | 2.3 |
| Cilia and flagella associated protein 126 | 2.2 |
| Actin, alpha 1a, skeletal muscle | 2.2 |
| FAD-dependent oxidoreductase domain containing 2 | 2.2 |
| Angiopoietin-like 2b | 2.1 |
| Transgelin | 2.1 |
| DnaJ (Hsp40) homolog, subfamily C, member 28 | 1.8 |
| THAP domain containing 5 | 1.7 |
| Downregulated genes | |
| Cytochrome P450, family 8, subfamily B, polypeptide 3 | 8.7 |
| Angiotensinogen | 6.5 |
| Small integral membrane protein 1 | 6.1 |
| Growth arrest and DNA-damage-inducible, beta a | 4.5 |
| Ephrin-A3b | 4.4 |
| Zinc finger protein 1065 | 4.3 |
| Chemokine (C-X-C motif) receptor 5 | 4.2 |
| Subcommissural organ spondin | 4.0 |
| Dachshund b | 3.9 |
| Tubulin tyrosine ligase-like family, member 12 | 3.8 |
| ATPase, class I, type 8B, member 5a | 3.4 |
| Forkhead box F2b | 3.4 |
| Potassium voltage-gated channel, Shaw-related subfamily, member 1b | 3.3 |
| Proteoglycan 4b | 3.3 |
| Zinc finger protein 1059 | 3.2 |
| Tumor necrosis factor receptor superfamily, member 21 | 3.1 |
| Growth arrest and DNA-damage-inducible, alpha, b | 3.1 |
| CCAAT/enhancer binding protein (C/EBP), delta | 3.1 |
| Carboxypeptidase A4 | 3.0 |
| Elastase 3 like | 2.9 |
| Mitogen-activated protein kinase kinase kinase 1 | 2.9 |
| TBC1 domain family, member 12a | 2.8 |
| Danio rerio serum/glucocorticoid regulated kinase | 2.6 |
| D. rerio endothelin 1 | 2.6 |
| D. rerio Smith–Magenis syndrome chromosome region, candidate 8b | 2.5 |
| (B) TOP 25-DEGs glucans-SVCV vs. PBS-SVCV | |
| Upregulated genes | |
| Tryptophan 2,3-dioxygenase a | 21.8 |
| Interferon, gamma 1-2 | 6.2 |
| Fatty acid binding protein 1b, liver, tandem duplicate 2 | 4.8 |
| Cytochrome P450, family 3, subfamily A, polypeptide 65 | 4.5 |
| Geminin, DNA replication inhibitor | 4.4 |
| Microsomal triglyceride transfer protein | 4.3 |
| Inositol 1,4,5-trisphosphate receptor-interacting protein | 4.2 |
| Heparanase | 4.2 |
| Tumor protein p63 regulated 1-like | 4.2 |
| Receptor-type tyrosine-protein phosphatase S precursor | 3.7 |
| Major facilitator superfamily domain containing 2ab | 3.4 |
| RAS (RAD and GEM)-like GTP-binding 1 | 3.2 |
| Homogentisate 1,2-dioxygenase | 3.2 |
| ISG15 ubiquitin-like modifier | 3.1 |
| TSC22 domain family, member 3 | 3.0 |
| UDP glucuronosyltransferase 1 family polypeptide a1 | 3.0 |
| T-cell activation RhoGTPase activating protein b | 3.0 |
| VHSV-inducible protein—Salmo salar | 2.9 |
| UDP glucuronosyltransferase 1 family, polypeptide A7 | 2.9 |
| Interferon-induced very large GTPase 1-like | 2.8 |
| Mastermind-like transcriptional coactivator 3 | 2.7 |
| Forkhead box A2 | 2.7 |
| Complement component bfb | 2.7 |
| Cysteine-serine-rich nuclear protein 1a | 2.6 |
| Interferon regulatory factor 1b | 2.6 |
| Downregulated genes | |
| Leucine-rich repeat and Ig domain containing 4a | 45.8 |
| Cyclin Y | 29.2 |
| Desumoylating isopeptidase 1a | 27.3 |
| GTPase IMAP family member 4 | 26.5 |
| Leucine-rich repeat, immunoglobulin-like and transmembrane domains 1b | 25.4 |
| Gap junction alpha-1 protein-like | 23.1 |
| Vomeronasal 2 receptor, c2 | 22.4 |
| Motilin receptor | 20.0 |
| Membrane-bound O-acyltransferase domain containing 4 | 19.8 |
| Neural cell adhesion molecule 2 | 17.7 |
| Hyperpolarization activated cyclic nucleotide-gated potassium channel 4 | 16.2 |
| Transmembrane protein 63B | 14.7 |
| D. rerio neuropeptide FF receptor 1-like | 14.7 |
| Semaphorin 7A, GPI membrane anchor | 14.6 |
| Transmembrane protein 237a | 14.3 |
| Protein phosphatase 1, regulatory subunit 3Aa | 14.2 |
| Toll-like receptor 1 | 14.1 |
| Ceramide synthase 2-like | 13.7 |
| Lysine (K)-specific methyltransferase 2E | 13.4 |
| Uridine-cytidine kinase 1 | 13.3 |
| Parathyroid hormone 1 receptor a | 13.2 |
| Zinc finger, MYND-type containing 12 | 13.2 |
| FEV (ETS oncogene family) | 12.9 |
| Pyruvate dehydrogenase kinase, isozyme 3a | 12.5 |
| finTRIM family, member 58 | 12.5 |