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. 2018 Jul 9;9:1569. doi: 10.3389/fimmu.2018.01569

Figure 2.

Figure 2

Trypanosoma cruzi infection inhibits fibroblasts proliferation and induces an accumulation of senescence-associated heterochromatin foci (SAHF). NIH-3T3 fibroblasts were infected with culture-derived T. cruzi trypomastigotes (MOI 5:1) overnight followed by a washing step to eliminate extracellular parasites. (A) Assessment of cellular proliferative capacity in CFSE-labeled fibroblasts 3 days (3d) post-infection and before infection (0d) by flow cytometry. Fibroblasts were first gated based on FSC-A versus SSC-A parameters, and then CFSE staining was analyzed. (B) T. cruzi-infected and non-infected NIH-3T3 fibroblasts stained with DAPI (upper panels, 20× magnification) 3 days post-infection. Enlarged images of DAPI staining are shown in the lower panels (40× magnification). The fluorescence images were obtained and photographed from multiple fields, by using an Axioplan II microscope (Zeiss) and image pro-plus software version 7.0.1. Scale bars are equal to 20 µm. Data are representative of at least three independent experiments with similar results. Abbreviations: MOI, multiplicity of infection; Ctrl, control; CFSE, carboxyfluorsecein diacetate succinimidyl ester.