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(A) Schematic of the expected outcomes during an IVT that has a fixed ratio of thG:GTP and was peformed using either T7RNAP WT or P266L mutant. This illustration depicts qualitatively the gains with respect to abortive transcription (also, see Figure S2). (B) Comparison of a typical IVT versus the one-pot multi-enzyme approach designed to eliminate 5'-GTP-initiated RNAs.
