Figure 2.

Experimental LIESS–FCS recordings of free diffusion in SLBs (Abberior Star Red labeled DPPE in DOPC/cholesterol). (A, B) Representative correlation carpets of confocal (d_conf = 240 nm, upper panel) and STED (d_STED = 100 nm, lower panel) from (A) conventional sSTED–FCS and (B) LIESS–FCS recordings (measurement time of 150 s and 1.36 μm scan). (C) Values of transit times (average and standard deviation of the mean as error bars) determined from confocal and STED correlation carpets of the sSTED–FCS (red) and LIESS–FCS (green) recordings (72 curves in confocal and STED), indicating no significant difference between sSTED– and LIESS–FCS. (D) Values of D_rat along the pixels of the scanned line resulting from the analysis of the LIESS–FCS correlation carpets and (E) frequency histogram indicating fluctuations around D_rat = 1.0, i.e., free diffusion (red line in panel D).