Fig. 5.

Effect of deleting lipids metabolism associated genes on 7-DHC production. a 7-DHC production in the control (SyBE_Sc0125XJ06) and strains with individual deletion of FLD1 and NEM1. b Relative transcription levels of the post-squalene pathway genes in strains SyBE_Sc0125XJ06 (control), SyBE_Sc0125XJ07 (ΔFLD1), and SyBE_Sc0125XJ08 (ΔNEM1). Cells were harvested at 30 h (ethanol consumption phase). The relative transcription level for each gene was quantified by Real-Time PCR and determined as 2^−ΔΔCt using gene ALG9 for normalization (data listed in the box). All data were from at least triplicate experiments. Significance levels of t test were determined for P < 0.05. Upregulated, downregulated genes, and genes without significant transcriptional difference are denoted in red, blue, and gray, respectively