Fig. 1.

βAR down-regulation is associated with increased GRK2 protein levels in the ischemic muscle. (A) Bar graph showing blood perfusion in the ischemic hindlimb in rats at 15 days after femoral artery resection (HI; n = 6) and in sham-operated rats (sham; n = 6) as assessed by dyed beads dilution method. Data are expressed as percentage of ischemic to nonischemic hindlimb. (B) Bar graphs showing total βAR density on skeletal muscle plasma membrane preparations from rats 15 days after femoral artery resection (HI; n = 6) and sham-operated rats (n = 6). (C) Representative immunoblots (upper panel) and densitometric analysis (lower panel) of multiple (n = 3, including two samples per group each) independent experiments showing GRK2/GAPDH protein levels in skeletal muscle lysates from the ischemic hindlimb of rats undergoing femoral artery resection (HI, n = 6) and from sham-operated rats (n = 6). GAPDH was used as a loading control. Data are expressed as mean ± S.E.M. *P < 0.05 versus sham.