Fig. 4.
Fenoterol selectively stimulates endothelial β2AR proangiogenic signaling in BAECs. (A, B) Representative immunoblots (upper panels) and densitometric quantitative analysis (lower panel) of multiple (n = 3) independent experiments to evaluate in BAECs unstimulated (Ns) or stimulated with fenoterol (Fen, 1 µM) for 15 minutes. (A) Akt phosphorylation levels on serine 473 (ser473p-Akt) and (B) eNOS phosphorylation levels on serine 1177 (ser1177p-eNOS) and total protein levels. Total Akt and total e-NOS served as loading controls, respectively. Before Fen stimulation, a group of cells was pretreated with selective β2AR antagonist ICI-118,551 (ICI, 10 µM). *P < 0.05 versus Ns.