Fig. 5.
Effects of GRK2 levels on β2AR phosphorylation and Akt and eNOS activation after fenoterol (Fen) stimulation in BAECs. (A–D) Representative immunoblots (upper) and densitometric analysis (bottom) of multiple independent experiments (n = 3) to evaluate: (A) β2AR phosphorylation levels on threonine 384 (thr384p-β2AR), (B) ser473p-Akt, and (C) ser1177p-eNOS, and (D) total protein levels of GRK2 in total lysates of BAECs infected with adenoviruses (Ad) encoding for GFP, GRK2, or βARKct. Cells were not stimulated Ns or stimulated with Fen (1 μM), respectively, for (A) 5 minutes or (B–D) 15 minutes. Total β2AR, total Akt, total eNOS and GAPDH served as loading controls. Data are expressed as mean ± S.E.M. *P < 0.05 versus GFP Ns; #P < 0.05 versus GFP Fen.