Fig. 6.

Long-term effects of fenoterol (Fen) stimulation on β₂AR function in BAECs. (A–D) Densitometric analysis of multiple independent experiments to evaluate (A) GRK2 protein levels and (B) ^thr384p-β₂AR, (C) ^ser473p-Akt, or (D) ^ser1177p-eNOS in total lysates of BAECs infected with adenoviruses (Ad) encoding for GFP, GRK2, or βARKct. Cells were not stimulated Ns or stimulated with Fen (1 μM), respectively, for 6 hours (A–D). GAPDH, total β₂AR, total Akt, and total eNOS served as loading controls, respectively. *P < 0.05 versus GFP Ns. ^#P < 0.05 versus GRK2.