Fig. 2.

Cytostatic effect of dinaciclib on in vitro cultured glioma cells (A) U87, U87-EGFR-WT, U87-EGFRviii, LNZ308, LN229, LN18, T98G, U373, and A172 cells were seeded at 60% confluence, allowed to attach overnight, and treated with dinaciclib (5.0 µM) for 24 hours. Control cells received an equivalent amount of DMSO. Apoptosis was analyzed by flow cytometry. Bar chart represents data from three independent experiments. (B) U87, U87-EGFRviii, LNZ308, U373, LN229, LN18, and A172 cells were seeded at 60% confluence, allowed to attach overnight, and treated with dinaciclib (2.5 µM) for 24 hours. Cells were stained with Alexa Flour 488 Phalloidin as described in Materials and Methods. Nuclei were stained with DAPI. Control cells received DMSO. Morphologic and nuclear changes in response to inhibitor treatment were evaluated by microscopic inspection. (C) U87, U87-EGFRviii, LNZ308, U373, LN229, LN18, and A172 cells were seeded at 60% confluence, allowed to attach overnight, and treated with dinaciclib (2.5 µM) for 24 hours. Cell-cycle analysis using PI staining was performed as described in Materials and Methods. Results represent the mean of three independent experiments.