Fig. 4.

Effect of dinaciclib and ABT-737 on the cell-cycle profile and the expression levels of cell-cycle regulatory proteins. (A and B) Logarithmically growing U87, U87-EGFRviii, LNZ308 (upper panel), LN229, LN18, and T98G (lower panel) cells were treated with dinaciclib (indicated concentrations) or ABT-737 (100 nM) or the combination of both for 24 hours. Control cells received equivalent concentrations of vehicle, DMSO. Whole-cell extracts were prepared, and equal amounts of protein were separated by SDS-PAGE and subjected to Western blotting analysis with the indicated antibodies. β-actin served as loading control (A). Total Rb served as loading control (B). The results of a representative study are shown; two additional experiments produced similar results.