Table 1.
Primary antibodies used in our RPPA experiments.
| Antibody | Manufacturer | Catalogue number | Species | Dilution |
|---|---|---|---|---|
| AKT | Cell Signaling | 9272 | Rabbit | 1:3000 |
| AKT (S473) | Cell Signaling | 9271 | Rabbit | 1:250 |
| AKT (T308) | Cell Signaling | 9275 | Rabbit | 1:500 |
| AKT2 (5B5) | Cell Signaling | 2964 | Rabbit | 1:50 |
| C-Raf | Millipore | 04-739 | Rabbit | 1:250 |
| C-Raf (S338) 56A6) | Cell Signaling | 9427 | Rabbit | 1:200 |
| EGFR | Santa Cruz | SC-03 | Rabbit | 1:100 |
| EGFR (Y1173) | Epitomics | 1124 | Rabbit | 1:50 |
| EGFR (Y992) | Cell Signaling | 2235 | Rabbit | 1:100 |
| EGFY (Y1068) | Cell Signaling | 2234 | Rabbit | 1:100 |
| MAPK – ERK 1/2 | Cell Signaling | 9102 | Rabbit | 1:200 |
| HER2 | Lab Vision | MS-325-P1 | Mouse | 1:1000 |
| HER2 (Y1248) | Upstate | 06-229 | Rabbit | 1:750 |
| HER3 | Santa Cruz | 285 | Rabbit | 1:500 |
| HER3 (Y1289) | Cell Signaling | 4791 | Rabbit | 1:50 |
| HER4 | Cell Signaling | 4795S | Rabbit | 1:50 |
| MAPK (T202/Y204) - ERK 1/2 | Cell Signaling | 4377 | Rabbit | 1:1200 |
| MEK1 | Epitomics | 1235-1 | Rabbit | 1:1200 |
| MEK1/2 (S217/S221) | Cell Signaling | 9121 | Rabbit | 1:1000 |
| mTOR | Cell Signaling | 2972 | Rabbit | 1:400 |
| mTOR (S2448) | Cell Signaling | 2971 | Rabbit | 1:100 |
| mTOR (S2481) | Cell Signaling | 2974 | Rabbit | 1:100 |
| NF-kB-p65 (S536) | Cell Signaling | 3033 | Rabbit | 1:100 |
| p38_MAPK | Cell Signaling | 9212 | Rabbit | 1:300 |
| p38 MAP Kinase (T180/Y182) | Cell Signaling | 9211 | Rabbit | 1:250 |
| p70 S6 Kinase | Epitomics | 1494-1 | Rabbit | 1:250 |
| p70 S6 Kinase (T389) | Cell Signaling | 9205 | Rabbit | 1:250 |
| PDK1 (S241) | Cell Signaling | 3061 | Rabbit | 1:100 |
| PI3K-p110-alpha | Cell Signaling | 4255 | Rabbit | 1:100 |
| PTEN | Cell Signaling | 9552 | Rabbit | 1:1000 |
| S6 Ribosomal Protein (S235/S236) (2F9) | Cell Signaling | 2211 | Rabbit | 1:200 |
| S6 Ribosomal Protein (S240/S244) | Cell Signaling | 2215 | Rabbit | 1:3000 |
| SRC | Upstate | 05-184 | Mouse | 1:200 |
| SRC (Y527) | Cell Signaling | 2105 | Rabbit | 1:400 |
HER, human epidermal growth factor-like receptor; PTEN, phosphatase and tensin homolog; RPPA, reverse phase protein array.