Table 1.
Sequences of primers used for amplification.
| Gene | Forward primer | Reverse primer | Anneal. Temp. | PCR product size | Ref. |
|---|---|---|---|---|---|
| Caspase-3 | 5-’AAATTCAAGGGACGGGTCAT-3’ | 5’-ATTGACACAATACACGGGATCTGT-3’ | 55 | 225 | Liu et al. (2002)28 |
| Bax | 5’-AGGATGATTGCTGATGTGGATAC-3’ | 5’-CACAAAGATGGTCACTGTCTGC-3’ | 56 | 300 | Liu et al. (2002)28 |
| TNF-α | 5’-CCACCACGCTCTTCTGTCTAC-3’ | 5’-ACCACCAGTTGGTTGTCTTTG-3’ | 58 | 256 | Liu et al. (2002)28 |
| GAPDH | 5′- CAAGGTCATCCATGACAACTTTG -3′ | 5′- GTCCACCACCCTGTTGCTGTAG -3′ | 58 | 496 | Wiame et al. (2000)29 |
PCR: polymerase chain reaction; TNF: tumour necrosis factor; GAPDH: glyceraldehyde 3-phosphate dehydrogenase.