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. Author manuscript; available in PMC: 2018 Jul 16.
Published in final edited form as: Nature. 2017 Aug 30;549(7672):389–393. doi: 10.1038/nature23672

Figure 3. ERK activation in BRAFV600E microglia.

Figure 3

(a) CD68, YFP and pERK staining in spinal cord from 7-month old mice. Scale bars=500µm, 10µm for insets. n=4 per group. (b) pERK+ microglia in brainstem. Circles represent individual mice. One-way ANOVA. (c) ERK phosphorylation in spinal cords and brains from 6–9 month-old mice. Top: representative western blot, bottom: pERK/ERK ratio, n=5 per group. One-way ANOVA. (d) pERK expression in YFP+ microglia from BRAFVE mice. n=5 per group. Scale bars=5µm. (e) Numbers of microglia from 5–9 month-old mice Circles represent individual mice. One-way ANOVA. (f) Heatmap representation of cell frequency among CD45+ cells in the brain. n=3 per group. (g) Ki67+ and cleaved Caspase 3+ (Casp3) expression in YFP+ microglia from 5–9 month-old BRAFVE mice, n=6 per group. Unpaired two-tailed t-test. See also Extended data Fig. 7.