Table 1.
Details of All Quantitative PCR Primers
| Primer | Sequence or Manufacturer Code |
|---|---|
| Genotyping | |
| IRKO (forward 1) | TTAAGGGCCAGCTCATTCCTCC |
| IRKO (forward 2) | AGCTGTGCACTTCCCTGCTCAC |
| IRKO (reverse) | TCTTTGCCTGTGCTCCACTCTCA |
| hIRECO (forward) | ACGTCAGTAGTCATAGGAACTG-CGGTCG |
| hIRECO (reverse) | TGCCTTGATTCACCAGATGCTG-AGG |
| Quantitative RT-PCR | |
| Human insulin receptor (forward) | TGCCACCAACCCCTCTGT |
| Human insulin receptor (reverse) | CGGAGGGTGGTTTCCACTT |
| Murine β-actin (forward), for normalization of human insulin receptor | CGTGAAAAGATGACCCAGATCA |
| Murine β-actin (reverse), for normalization of human insulin receptor | TGGTACGACCAGAGGCATACAG |
| Murine insulin receptor | TaqMan probe (Thermo Fisher, Waltham, MA) Mm_01211875_m1 |
| Murine β-actin, for normalization of murine insulin receptor | TaqMan probe (Thermo Fisher) Mm_00607939 |