Fig. 2.

Metabolites related to glycolysis/gluconeogenesis, TCA cycle, energy carriers, and creatine metabolism (see Supplementary Table 1). The relative levels of the annotated metabolites are represented as bar graphs (open column, BAT of rats kept at room temperature: closed column, the BAT of rats exposed to the cold temperature). N.D. indicates “not detected.” Height of column with large quantity is set to 100%, and that for the smaller quantity is shown proportionally. Numbers on the columns represent the relative levels of metabolites from the BAT of rats exposed to the cold compared to those from the BAT of rats kept at room temperature. <1 indicates that the value for the BAT of rats exposed to cold is below the detection limit. The abbreviations are as follows: G1P, glucose 1-phosphate; G6P, glucose 6-phosphate; F6P, fructose 6-phosphate; F1,6P, fructose 1,6-diphosphate; DHAP, dihydroxyacetone phosphate; GAP, glyceraldehyde 3-phosphate; 1,3-DPG, 1,3-diphosphoglycerate; 3-PG, 3-phosphoglycerate; 2-PG, 2-phosphoglycerate; PEP, phosphoenolpyruvate; ADP, adenosine diphosphate; ATP, adenosine triphosphate; NAD, nicotinamide adenine dinucleotide; NADH, nicotinamide adenine dinucleotide phosphate; NADP, nicotinamide adenine dinucleotide phosphate; NADPH, reduced nicotinamide adenine dinucleotide phosphate; and 4-GBA, 4-guanidinobutyric acid.

Metabolites related to glycolysis/gluconeogenesis, TCA cycle, energy carriers, and creatine metabolism (see Supplementary Table 1). The relative levels of the annotated metabolites are represented as bar graphs (open column, BAT of rats kept at room temperature: closed column, the BAT of rats exposed to the cold temperature). N.D. indicates “not detected.” Height of column with large quantity is set to 100%, and that for the smaller quantity is shown proportionally. Numbers on the columns represent the relative levels of metabolites from the BAT of rats exposed to the cold compared to those from the BAT of rats kept at room temperature. <1 indicates that the value for the BAT of rats exposed to cold is below the detection limit. The abbreviations are as follows: G1P, glucose 1-phosphate; G6P, glucose 6-phosphate; F6P, fructose 6-phosphate; F1,6P, fructose 1,6-diphosphate; DHAP, dihydroxyacetone phosphate; GAP, glyceraldehyde 3-phosphate; 1,3-DPG, 1,3-diphosphoglycerate; 3-PG, 3-phosphoglycerate; 2-PG, 2-phosphoglycerate; PEP, phosphoenolpyruvate; ADP, adenosine diphosphate; ATP, adenosine triphosphate; NAD, nicotinamide adenine dinucleotide; NADH, nicotinamide adenine dinucleotide phosphate; NADP, nicotinamide adenine dinucleotide phosphate; NADPH, reduced nicotinamide adenine dinucleotide phosphate; and 4-GBA, 4-guanidinobutyric acid.