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. 2018 Jun 29;9(50):29286–29303. doi: 10.18632/oncotarget.25389

Figure 7. Involvement of Akt in regulating CXCL2 in BT549 cells.

Figure 7

(A) Effects of MK2206 (Akt inhibitor) on Akt activation in BT549 cells. β-actin was used as the loading control. (B) Effects of MK2206 on basal and TNF-induced levels of CXCL2 promoter activity. (C) Effects of PD98059 (PD, Erk inhibitor) on basal and TNF-induced levels of CXCL2 promoter activity. (D) Effects of Akt and Erk knockdown on CXCL2 promoter activity. Knockdown of Akt and Erk was confirmed by western blots, with β-actin as the loading control. (E) Effects of Akt1 overexpression on basal and TNF-induced levels of CXCL2 promoter activity. Overexpression of Akt1 was confirmed by western blots, with β-actin as the loading control. The asterisks (*,**) and hash (#) indicate a statistically significant increase and decrease (p ≤ 0.05) as determined by ANOVA and Tukey's pairwise comparison, respectively. The Student's t-test was also performed for values marked with a horizontal line. EV: empty expression vector, Akt1-EV: Akt1 expression vector.