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. Author manuscript; available in PMC: 2018 Jul 16.
Published in final edited form as: Cancer Res. 2017 Jul 18;77(18):4921–4933. doi: 10.1158/0008-5472.CAN-16-3413

Figure 2. USP9X stabilizes upstream Hippo components.

Figure 2

A, Knockdown of USP9X by siRNA in HPDE6 (left) and MCF10A (right) cells resulted in downregulation of LATS, WW45, and phospho-YAP. Down-regulation of KIBRA was seen in MCF10A cells. B, Screening of USP9X shRNA in MCF10A cells. Co-downregulation of LATS with USP9X was seen with shRNA #3 and #4. C, MCF10A were stably knocked down with USP9X shRNA #3. LATS, WW45, KIBRA, and phospho-YAP were downregulated (left panel) and TAZ was significantly translocated to the nucleus (right panel) in response to USP9X silencing. D, LATS was downregulated in Floxed USP9X MEF cells after Cre expression. E, F, Knockdown of USP9X by siRNA (E) and shRNA (F) in various cell lines resulted in upregulation of YAP/TAZ target genes, CTGF and CYR61 as accessed by real-time PCR.