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. Author manuscript; available in PMC: 2018 Jul 16.
Published in final edited form as: Cancer Res. 2017 Jul 18;77(18):4921–4933. doi: 10.1158/0008-5472.CAN-16-3413

Figure 5. YAP/TAZ and TEAD are downstream mediators of USP9X.

Figure 5

A, The activation of YAP/TAZ target genes, CTGF and Cyr61, due to USP9X knockdown could be rescued by knockdown of YAP/TAZ. The mRNAs of CTGF and Cyr61 were accessed by RT-PCR. B, The enhanced anchorage-independent growth by the USP9X knockdown in MCF7 cells could be rescued by knockdown of YAP and TAZ. C, D, TEAD2 was identified as one of the major TEAD isoforms downstream of USP9X. The activation of YAP/TAZ target genes, CTGF and Cyr61, due to USP9X knockdown could be rescued by knockdown of TEAD2. siRNA of the four TEAD isoforms could be effectively knocked down in cells (C) and TEAD2 knockdown could significantly suppress increased CTGF and Cyr61 mRNA in USP9X knockdown cells (D). E, TEAD2 is able to rescue the anchorage-independent growth of USP9X knockdown cells. F, LATS2 is able to rescue the YAP and TAZ expression induced by the USP9X knockdown.