Table 4. Wnt Pathway Components as Targets of Kinases Involved in the Regulation of cell cycle.

Substrate	Kinase	Modified Residue	Function	Method of Detection1	Species2	Ref.
β-catenin	NEK2	S33/S37/T41/T102/T556/S675	β-catenin stabilization, presence at mitotic centrosomes, promotion of centrosome disjunction, regulated upstream by Plk1	MS, WB	h	(Mbom et al., 2014)
APC	Bub1–Bub3, BubR1–Bub3 complexes	middle and C-terminal fragments	chromosome segragation, regulation of kinetochore-microtubule attachment (speculation)	IVP	h	(Kaplan et al., 2001)
Dsh	NEK2	Dsh 1–340 and Dsh 166–623 fragments	activation of Wnt/β-catenin signalling, regulation of Dsh half-life	IVP	d	(Schertel et al., 2013)
DVL	NEK2	T15/S280/S643/S679	separation of centrosomes, disengagement of centrosomal linker	MS,IVP, WB, IF	h	(Cervenka et al., 2016)
DVL2	PLK1	T206	spindle orientation, MT-KT attachment, SAC activation	IVP, WB	h	(Kikuchi et al., 2010)
LRP6	Cyclin Y/Pftk1	S1490	cell cycle dependent Wnt signalling activity	WB, IF	d, x, h	(Hadjihannas, Bruckner, and Behrens, 2010)
Axin	PLK1	S157	Axin-y-tubulin interaction, centrosome formation and segregation	MS, WB	h	(Mick et al., 2015)

¹Methods of detection: MS (mass spectrometry), WB (Western Blotting), IVP (in vitro phosphorylation), IF (immunofluorescence with phospho-specific antibodies)

²Species: h – human, d – Drosophila, x - Xenopus