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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: Methods. 2017 Dec 30;137:11–19. doi: 10.1016/j.ymeth.2017.12.003

Fig. 1.

Fig. 1

AAP-mediated regulation of mRNA translation in response to Arg in the N. crassa CFPS examined by measurement of luciferase reporter activity. Triplicate reactions were programmed with the indicated mRNAs and luciferase activity levels measured after 30 min of translation as described in the text. Reactions contained low Arg (black) or high Arg (gray). Amounts of luciferase produced, normalized to the amount produced from the control fLuc mRNA (lacking any known 5′-regulatory sequences) in low Arg, are shown. Plasmids used [27–29] to generate the mRNAs were: fLuc, pPQ101 (originally called pHLucNFS); AAPw, pPR301 (wild- type AAP as uORF); AAPm, pPS301 (non-regulatory D12N mutant AAP as uORF); AAPw Fusion, pKL105 (wild-type AAP fused in frame with luciferase); AAPm Fusion, pKLS105 (D12N mutant AAP fused in frame with luciferase). Error bars represent SD values.