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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: Methods. 2017 Dec 30;137:11–19. doi: 10.1016/j.ymeth.2017.12.003
Component Final concentration Stock Amount stock/1.75 ml
HEPES-KOH, pH 7.6 10 mM 1 M 17.5 μl
DTT 1 mM 2 M 0.875 μl
Creatine phosphokinase 10 U/μl Sigma C3755 17,500 U
Glycerol 50% 875 μl

Add DEPC-treated water to 1.75 ml, mix, prepare 25 μl aliquots, freeze in liquid nitrogen, store at −80 °C.