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| Component | Final concentration | Stock | Amount stock/3 L |
|---|---|---|---|
| KNO3 | 6 g | ||
| K2HPO | 4.2 g | ||
| KH2PO4 | 3 g | ||
| MgSO4·7H2O | 3 g | ||
| NaCl | 0.6 g | ||
| H3BO3 | 0.6 g | ||
| CaCl2·2H2O | 0.05 g | ||
| Trace element Solution | 0.6 ml | ||
| Biotin solution | 0.1 mg/ml | 0.3 ml |
Components are dissolved consecutively in a large Erlenmeyer flask, with stirring and moderate heating. CaCl2 is dissolved separately in 20 ml of water and added slowly to the stock. Add water to 3 L, mix, add 2 ml of chloroform as preservative, store at 4 °C.
