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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: Methods. 2017 Dec 30;137:11–19. doi: 10.1016/j.ymeth.2017.12.003
Component Final concentration Stock Amount stock/100 ml
SC salt 0.1× 2× 5 ml
Sucrose 0.5% 0.5 g
Agar 2% 2 g
Sodium iodoacetate 1 mM 100 mM 1 ml

Add water to 99 ml, sterilize by autoclaving. Sodium iodoacetate is freshly prepared in water, sterilized by filtration, and added to the autoclaved medium cooled to 55 °C