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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: Methods. 2017 Dec 30;137:11–19. doi: 10.1016/j.ymeth.2017.12.003
Component Final concentration Stock Amount Stock/250 ml
Vogel’s minimal salt 1× 50× 5 ml
Sucrose 2% 5 g
Agar 2% 5 g
FGS 1× 10× 2.5 ml

Add water to 247.5 ml, sterilize by autoclaving. FGS is added to the autoclaved medium cooled to 55 °C.