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| Component | Final concentration | Stock | Amount stock/L |
|---|---|---|---|
| HEPES-KOH, pH 7.6 | 30 mM | 1 M | 30 ml |
| KOAc, pH 7.0 | 100 mM | 4 M | 25 ml |
| Mg(OAc)2, pH 7.0 | 3 mM | 0.3 M | 10 ml |
| DTT | 2 mM | 2 M | 1 ml |
Add DEPC-treated water to 1 L, mix, store at 4 °C. DTT is added immediately prior to use.
