Component	Final concentration	Stock	Amount stock/ml
APMSF	1.25 mg/ml	25 mg/ml
Pepstatin A	0.25 mg/ml	5 mg/ml
Antipain	0.25 mg/ml	5 mg/ml
Chymostatin	0.25 mg/ml	5 mg/ml
Elastatinal	0.05 mg/ml	1 mg/ml
Leupeptin	0.25 mg/ml	5 mg/ml

Dissolve 25 mg APMSF, 5 mg Pepstatin A, 5 mg Antipain, 5 mg Chymostatin, and 1 mg Elastatinal in DMSO to make 1 ml 1000× stock. Dissolve 5 mg leupeptin in DEPC-treat water to make 1 ml 1000× stock. Store both stocks at −80 °C. To make 50 × stock before use, take 50 μl of each stock, add DEPC-treated water to 1 ml. The remaining 50× stock is stored at −80 °C.