Fig 2. UV/vis spectrum recorded for the PpSB1-LOV-R66I in the light- and dark-states in solution.

The inset shows the characteristic absorption maxima at 447 nm after the dark state recovery. The same sample was measured in light- and dark-state. Please note that the difference in absorbance at ˜280 nm is insignificant as this is usually due to the flavin chromophore involved in the adduct formation.