Levels of IL-4, IL-5, IL-13, TGF-β and IL-10 in the lungs from WT and Chit1−/− mice after PBS or OVA challenge were evaluated. (A and C) The protein levels of IL-4, IL-5, IL-13, TGF-β and IL-10 in BAL fluid measured by ELISA. (B and D) The mRNA expression levels of IL-4, IL-5, IL-13, TGF-β and IL-10 measured by real-time RT-PCR. The values represent the mean±SEM of duplicate evaluations in a minimum of 5 mice in each group. *p<0.05, **p<0.01, ***p<0.001, n.s.=not significant.