Figure 6.

Proliferation and cytokine production of splenocytes derived from immunised mice. Splenocytes from mice immunised with PBS or formalin-inactivated EV71 alone or adjuvanted with CpG were harvested and cultured in medium containing 10 μg/mL heat-inactivated EV71. (a) After 5 days in culture, proliferation was measured as [³H]-thymidine incorporation. Culture supernatants were analysed after 2 days for IFN-γ (b), IL-4 (c), and IL-17 (d) levels in response to heat-inactivated EV71. Thymidine uptake was determined by harvesting cells and using a scintillation counter to measure the level of incorporation (as counts per minute [cpm]). Values shown are means ± SEMs of six mice in each experimental group; *p < 0.05. One representative study of three with similar results is shown.