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. 2018 Jul 16;8:10738. doi: 10.1038/s41598-018-29121-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Wound size is positively correlated with dispersal-induced septicemia. Treatment of 48-hour mouse chronic wounds of varying sizes (0.5, 1.0, and 1.5 cm in diameter) with 10% α-amylase and cellulase (1:1; GH) resulted in no mortality in the 0.5 cm group after 36 hours, and significantly increased mortality in the 1.0 cm and 1.5 cm groups, indicating that increased wound size is correlated with septicemia. A representative mouse for each wound size, before and after treatment, is shown on the right, with arrows indicating treatment. Repeated measures ANOVA and the Tukey-Kramer multiple-comparison test were used to test for differences between columns: *p < 0.05, **p < 0.01. N = 9 for each group.