Table 1.
Details of markers selected for the study and their amplified products.
| S. No. | ISSR Primers | Sequence (5′-3′) | Annealing temperature (°C) | Size range of Amplicons | No. of amplified bands | Polymorphic loci | Polymorphism (%) |
|---|---|---|---|---|---|---|---|
| 1. | ISSR5 | (GTG)5 | 52 | 200 bp–2.8 kb | 13 | 12 | 92.30 |
| 2. | ISSR7 | (AC)8G′ | 48 | 100 bp–2.9 kb | 17 | 17 | 100 |
| 3. | ISSR8 | (GA)8CT | 52 | 150 bp–2.8 kb | 15 | 15 | 100 |
| 4. | ISSR10 | (CA)7CC | 48 | 200 bp–2.5 kb | 11 | 9 | 81.82 |
| 5. | ISSR11 | (CA)7CG | 48 | 100 bp–2 kb | 10 | 9 | 90 |
| 6. | ISSR13 | (GT)7CG | 48 | 250 bp–2.8 kb | 9 | 8 | 88.89 |
| 7. | ISSR16 | (GTG)4GAC | 52 | 200 bp–2.1 kb | 9 | 9 | 100 |
| 8. | ISSR17 | (AG)8G | 49.5 | 350 bp–2.1 kb | 11 | 8 | 72.73 |
| 9. | ISSR21 | (TC)8C | 49.5 | 250 bp–2 kb | 11 | 11 | 100 |
| 10. | ISSR22 | (TC)8G | 49.5 | 300 bp–1.1 kb | 13 | 10 | 76.92 |
| 11. | ISSR25 | (GA)8GT | 49.5 | 250 bp–3 kb | 11 | 10 | 90.91 |
| Total | 130 | 118 | 90.76 |