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. 2018 Jul 16;8:10690. doi: 10.1038/s41598-018-29112-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of 1 µg/ml of dentin matrix components (DMCs) treated with matrix metalloproteinases (MMPs) on wound scratch (B–H) and chemotactic (I) cell migration. Eight points were randomly selected to assess wound scratch migration; the distances of the cells from the edge of the scratch wound were measured by microscopic observation (A). Wound scratch migration of cells incubated with 1 µg/ml DMCs treated with MMP-1 (B), MMP-2 (C), MMP-3 (D), MMP-8 (E), MMP-9 (F), MMP-13 (G) and MMP-20 (H) is presented as the amount of movement, and chemotactic migration of cells incubated with 1 µg/ml DMCs treated with MMP-1, MMP-2, -3, -8, -9, -13, and -20 is presented as relative fluorescence units (RFU) (I). One microgram per millilitre of DMCs treated with MMP-1, -3, or -9 promoted horizontal migration, compared with 1 µg/ml of incubated DMCs without the addition of MMPs (p < 0.05). The trans-well assay demonstrated the significant chemotactic effects of 1 µg/ml of DMCs treated with MMP-1, -3, -9, or -20, compared with the experimental and negative controls (p < 0.05). Significant differences were not observed with 1 µg/ml DMCs treated with other MMPs, MMPs incubated alone or at lower concentrations (0.01–0.1 µg/ml) of the above-mentioned MMPs. Groups with similar lower-case letters (i.e., a and b) are not significantly different. Data represent five independent experiments.