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. 2018 Jul 16;8:10690. doi: 10.1038/s41598-018-29112-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of 1 µg/ml of dentin matrix components (DMCs) treated with matrix metalloproteinase (MMP)-1 (A), MMP-2 (B), MMP-3 (C), MMP-8 (D), MMP-9 (E), MMP-13 (F) and MMP-20 (G) on cell proliferation of rat primary pulp cells. Pulp cell proliferation was promoted by 1 µg/ml of DMCs treated with MMP-1 (A), -8 (D), -9 (E), or -13 (F), compared with incubated DMCs without the addition of MMPs (p < 0.05). Significant differences were not observed with 1 µg/ml DMCs treated with other MMPs, MMPs incubated alone or at lower concentrations (0.01–0.1 µg/ml) of the above mentioned MMPs (Supplementary Figure 6). Groups with similar lower-case letters (i.e., a and b) are not significantly different. Data represent five independent experiments.