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. 2018 Jul 16;8:10690. doi: 10.1038/s41598-018-29112-3

Figure 6.

Figure 6

Effects of 1 µg/ml of dentin matrix components (DMCs) treated with matrix metalloproteinase (MMP)-1 (A), MMP-2 (B), MMP-3 (C), MMP-8 (D), MMP-9 (E), MMP-13 (E), or MMP-20 (F) on mineralized nodule formation in rat primary pulp cells. Cells were exposed to 1 µg/ml of MMP-treated DMCs, incubated DMCs without MMPs or medium alone containing 50 μg/ml of ascorbic acid and 10 mm of β-glycerophosphate for 21 days. DMCs (1 µg/ml) treated with MMP-1 (A), -9 (E), -13 (F), or -20 (G) significantly promoted mineralized nodule formation in pulp cell cultures after 21 days incubation (p < 0.05). Significant differences were not observed with DMCs treated with other MMPs, MMPs incubated alone or at lower concentrations (0.01–0.1 µg/ml) of the above-mentioned MMPs (Supplementary Figure 8). Groups with similar lower-case letters (i.e., a and b) are not significantly different. Data represent five independent experiments.