Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 6;11(6):748–757. doi: 10.14202/vetworld.2018.748-757

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Hosein, et al.

Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Figure-1 — (a) Universal polymerase chain reaction (PCR) using primer sequences targeting Erythritol catabolism, gene eryC (Lane 1:100 bp DNA ladder, Lane 2, 3, 4, 5: Brucella cultures (587 bp), Lane 6: Brucella lymph node DNA extract (587 bp), Lanes 7, 8: Brucella melitensis positive control (587 bp), Lane 9: Brucella abortus S19 control). (b): Universal PCR using primer sequences targeting immunodominant antigen, gene bp26 (Lane 1: 100 bp DNA ladder, Lane 2, 3, 4, 5, 6: Brucella cultures (450 bp), Lane 7: B. melitensis3 culture positive control (450 bp), Lane 8, 9: Brucella lymph node DNA extract, 450 bp). (c): Multiplex Bruce-ladder PCR of Brucella isolates (Lane 1: DNA ladder, Lane 2: B. abotus Bruce-ladder kit control (587 bp and 1682 bp), Lane 3: B. abortus RB51 Bruce-ladder kit control (587 bp and 2524 bp), Lane 4: Brucella suis Bruce-ladder kit control (272 bp, 587 bp, 1071 bp, and 1682 bp), Lane 5: B. melitensis Rev1 Bruce-ladder kit control (218 bp, 587 bp, 1071 bp, and 1682 bp), Lane 6: B. melitensis positive control (587 bp, 1071 bp, and 1682 bp), Lanes 7, 8: B. melitensis cultures (587 bp, 1071 bp, and 1682 bp).