Table-3.

Typing of six Brucella isolates recovered from buffaloes at species and biovar level.

Brucella isolates	CO2	H2S	Urease	Growth on dyes				Lysis by phage			Monospecific sera			Conclusion
				Thionin		Fuchsin		Tp		Iz1	A	M	R
				A	b	A	b	RTD	RTD 104	RTD
Field isolates (6)	-	-	+ in 20 h	+	+	+	+	-	-	+	+	+	-	B. melitensis 3
Reference strains
B. melitensis Ether	-	-	+ in 18-24 h	+	+	+	+	-	-	+	+	+	-	B. melitensis 3
B. abortus544	-	+	+ in 2 h	-	-	+	+	+	+	+	+	-	-	B. abortus 1
B. suis1330	-	+++	++ in <15 min	+	+	-	-	-	+	+	+	-	-	B. suis 1

RTD=Routine test dilution, Tp=Tbilisi, Iz₁=Izatnagar, a=1:50000, b=1:100000, A=Anti Brucella abortus, M:=Anti Brucella melitensis, R=Rough Brucella antiserum