Skip to main content
. 2018 Jun 10;11(6):782–788. doi: 10.14202/vetworld.2018.782-788

Table-1.

Sequence, annealing temperature, and fragment size of the pairs of primers used for RT-PCR.

Gene Primer sequence Annealing temp. (°C) Fragment size (bp) Accession no.
NF-kB p65 (RELA) F-TCCAAGTTCCCATAGAAGAGCA
R-TCCCAGAGTTCCGATTCACC
57 192 DQ355511
IL-6 F-ATGACGAGTGTGAAAGCAGC
R-TCGCCTGATTGAACCCAGAT
59 105 AY347710.1
TNF-a F-GGTCAACATCCTGTCTGCCA
R-ACTGAGGCGATCTCCCTTCT
59 130 XM_006041930.1
Interferon g F-CGCAAAGCCATAAATGAACTC
R-GGACCATTACGTTGATGCTC
60 120 AF484688.1

PCR=Polymerase chain reaction