Table-1.

Target genes, primer sequence ((5^’3) 5 with peT28c vector restriction sites (forward and reverse), amplicon length (bp), and NCBI accession number.

Target gene	Sequence of primers with vector	Amplicon length (bp)	NCBI accession no.
BSP1	For: 5’GATCGAATTCTTATGGCACTGCAGTTGGGGC3	440	NM_001001145.1
	Rev: 5’GATCAAGCTTCTAGCAATACTTCCAAGCTCTGTCCT3’
BSP3	For: 5’GATCGAATTCATATGGCACTGCGTTTGGGG3’	446	NM_174840.1
	Rev: 5’GATCAAGCTTCTAGCAATACTTCCAAACTCCATCCT3’
BSP5	For: 5’GATCGAATTCATATGGCACCGCTAGTGGGGC3’	575	NM_174842.2
	Rev: 5’GATCCTCGAGCTAGCAATACTTCCAAGCTTTATCCC 3’

BSP=Binders of sperm protein