Table 1.
S-plot proteins obtained from OPLS–DA regression analysis (p(corr) ± 0.80), of all stage I–II OPSCCs versus controls
| Primary accession | Peptide count | Unique peptides | Confidence score | ANOVA (p) | Max fold change | Highest mean | Lowest mean | Protein name | Gene name | p[1] | p(corr)[1] |
|---|---|---|---|---|---|---|---|---|---|---|---|
| P19105 | 2 | 2 | 10.19 | 0 | 184.10 | Stage I–II OPSCC | Control | Myosin regulatory light chain 12A | MYL12A | –0.04 | –0.95 |
| P01034 | 2 | 2 | 12.07 | 0 | Infinity | Stage I–II OPSCC | Control | Cystatin-C | CST3 | –0.02 | –0.95 |
| Q8N2Z9 | 3 | 3 | 15.95 | 0 | 14.92 | Stage I–II OPSCC | Control | Centromere protein S | APITD1 | –0.04 | –0.94 |
| Q09013;M0QX08 | 4 | 3 | 35.22 | 0 | 9.23 | Stage I–II OPSCC | Control | Myotonin-protein kinase | DMPK | –0.03 | –0.92 |
| P15428 | 2 | 2 | 11.07 | 0 | Infinity | Stage I–II OPSCC | Control | 15-hydroxyprostaglandin dehydrogenase [NAD(+)] | HPGD | –0.02 | –0.92 |
| Q8N3Y7 | 5 | 3 | 33.98 | 1.12E−10 | 10.36 | Stage I–II OPSCC | Control | Epidermal retinol dehydrogenase 2 | SDR16C5 | –0.03 | –0.91 |
| P36980 | 12 | 4 | 74.12 | 0 | 8.55 | Stage I–II OPSCC | Control | Complement factor H-related protein 2 | CFHR2 | –0.02 | –0.89 |
| A0A0B4J2B5 | 3 | 2 | 10.40 | 0 | 35.11 | Stage I–II OPSCC | Control | Protein IGHV3R16-9 (fragment) | IGHV3R16-9 | –0.01 | –0.87 |
| P48736 | 6 | 4 | 34.80 | 2.00E−14 | 8.29 | Stage I–II OPSCC | Control | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform | PIK3CG | –0.04 | –0.86 |
| Q3MJ40 | 3 | 2 | 24.36 | 1.11E−16 | 10.49 | Stage I–II OPSCC | Control | Coiled-coil domain-containing protein 144B | CCDC144B | –0.02 | –0.85 |
| O94887 | 7 | 3 | 36.29 | 3.27E−07 | 3.80 | Stage I–II OPSCC | Control | FERM, RhoGEF and pleckstrin domain-containing protein 2 | FARP2 | –0.01 | –0.81 |
| Q9C091 | 8 | 6 | 43.13 | 0 | 9.39 | Stage I–II OPSCC | Control | GREB1-like protein | GREB1L | –0.03 | –0.81 |
| A0A1B0GUA9 | 2 | 2 | 11.10 | 0 | Infinity | Stage I–II OPSCC | Control | Uncharacterised protein | N/A | –0.02 | –0.80 |
| P07357 | 39 | 37 | 309.89 | 6.18E−07 | 1.69 | Control | Stage I–II OPSCC | Complement component C8 alpha chain | C8A | 0.06 | 0.80 |
| P05452 | 19 | 17 | 183.04 | 1.11E−07 | 1.52 | Control | Stage I–II OPSCC | Tetranectin | CLEC3B | 0.04 | 0.80 |
| P02753 | 48 | 42 | 241.90 | 2.55E−07 | 2.03 | Control | Stage I–II OPSCC | Retinol-binding protein 4 | RBP4 | 0.09 | 0.81 |
| P07996 | 26 | 24 | 196.30 | 1.55E−09 | 3.15 | Control | Stage I–II OPSCC | Thrombospondin-1 | THBS1 | 0.06 | 0.81 |
| P05543 | 20 | 18 | 142.13 | 3.40E−07 | 2.40 | Control | Stage I–II OPSCC | Thyroxine-binding globulin | SERPINA7 | 0.05 | 0.82 |
| P01019 | 81 | 76 | 374.07 | 7.15E−09 | 2.58 | Control | Stage I–II OPSCC | Angiotensinogen | AGT | 0.14 | 0.84 |
| Q6PI47 | 3 | 3 | 22.91 | 2.59E−08 | 2.44 | Control | Stage I–II OPSCC | BTB/PZ domain-containing protein KCTD18 | KCTD18 | 0.06 | 0.85 |
| Q7Z6K3 | 3 | 3 | 29.13 | 6.27E−09 | 6.48 | Control | Stage I–II OPSCC | Protein prenyltransferase alpha subunit repeat-containing protein 1 | PTAR1 | 0.03 | 0.85 |
| P00734 | 106 | 90 | 686.35 | 1.45E−09 | 1.82 | Control | Stage I–II OPSCC | Prothrombin | F2 | 0.13 | 0.85 |
| O14791 | 11 | 6 | 64.95 | 2.10E−08 | 3.10 | Control | Stage I–II OPSCC | Apolipoprotein L1 | APL1 | 0.01 | 0.85 |
| P03952 | 53 | 44 | 476.60 | 4.74E−10 | 1.63 | Control | Stage I–II OPSCC | Plasma kallikrein | KLKB1 | 0.07 | 0.85 |
| P42336 | 9 | 3 | 45.78 | 8.43E−09 | 2.46 | Control | Stage I–II OPSCC | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform | PIK3CA | 0.01 | 0.86 |
| P43652 | 108 | 90 | 739.29 | 3.46E−09 | 1.88 | Control | Stage I–II OPSCC | Afamin | AFM | 0.12 | 0.86 |
| Q5VU65 | 5 | 3 | 22.62 | 1.67E−10 | 6.46 | Control | Stage I–II OPSCC | Nuclear pore membrane glycoprotein 210-like | NUP210L | 0.01 | 0.87 |
| P00740 | 16 | 14 | 113.09 | 7.90E−09 | 3.73 | Control | Stage I–II OPSCC | Coagulation factor IX | F9 | 0.04 | 0.87 |
| P04196 | 62 | 58 | 332.49 | 5.30E−09 | 2.62 | Control | Stage I–II OPSCC | Histidine-rich glycoprotein | HRG | 0.14 | 0.87 |
| P43251 | 7 | 6 | 49.04 | 1.04E−07 | 3.04 | Control | Stage I–II OPSCC | Biotinidase | BTD | 0.02 | 0.87 |
| P04004 | 83 | 65 | 503.49 | 1.96E−10 | 2.03 | Control | Stage I–II OPSCC | Vitronectin | VTN | 0.16 | 0.87 |
| Q8IVH4 | 3 | 3 | 26.12 | 9.95E−06 | 3.33 | Control | Stage I–II OPSCC | Methylmalonic aciduria type-A protein, mitochondrial | MMAA | 0.02 | 0.88 |
| P08185 | 27 | 23 | 212.28 | 7.43E−10 | 2.33 | Control | Stage I–II OPSCC | Corticosteroid-binding globulin | SERPINA6 | 0.07 | 0.88 |
| P02749 | 124 | 109 | 472.25 | 1.30E−10 | 2.15 | Control | Stage I–II OPSCC | Beta-2-glycoprotein 1 | APH | 0.27 | 0.88 |
| Q14624 | 138 | 123 | 984.03 | 4.93E−10 | 1.91 | Control | Stage I–II OPSCC | Inter-alpha-trypsin inhibitor heavy chain H4 | ITIH4 | 0.19 | 0.89 |
| P08697 | 38 | 36 | 326.03 | 4.46E−11 | 1.95 | Control | Stage I–II OPSCC | Alpha-2-antiplasmin | SERPINF2 | 0.08 | 0.89 |
| P00915 | 3 | 2 | 16.19 | 0 | Infinity | Control | Stage I–II OPSCC | Carbonic anhydrase 1 | CA1 | 0.01 | 0.89 |
| Q9UK55 | 8 | 7 | 43.36 | 0 | 8.09 | Control | Stage I–II OPSCC | Protein Z-dependent protease inhibitor | SERPINA10 | 0.08 | 0.89 |
| Q15102 | 2 | 2 | 10.00 | 0 | 1344.35 | Control | Stage I–II OPSCC | Platelet-activating factor acetylhydrolase IB subunit gamma | PAFAH1B3 | 0.01 | 0.89 |
| P20851 | 10 | 6 | 78.00 | 0 | 45.67 | Control | Stage I–II OPSCC | C4b-binding protein beta chain | C4BPB | 0.03 | 0.89 |
| P05090 | 24 | 23 | 200.64 | 2.42E−14 | 2.69 | Control | Stage I–II OPSCC | Apolipoprotein D | APD | 0.09 | 0.90 |
| P02774 | 206 | 181 | 1148.20 | 2.81E−11 | 1.79 | Control | Stage I–II OPSCC | Vitamin D-binding protein | GC | 0.31 | 0.90 |
| P19652 | 31 | 20 | 133.66 | 1.44E−15 | 6.38 | Control | Stage I–II OPSCC | Alpha-1-acid glycoprotein 2 | RM2 | 0.07 | 0.90 |
| Q52LA3 | 4 | 3 | 26.69 | 6.92E−09 | 19.54 | Control | Stage I–II OPSCC | Protein lin-52 homologue | LIN52 | 0.02 | 0.90 |
| P01591 | 3 | 2 | 21.76 | 0 | 20.46 | Control | Stage I–II OPSCC | Immunoglobulin J chain | JCHAIN | 0.01 | 0.91 |
| P02748 | 80 | 70 | 553.63 | 3.29E−11 | 2.21 | Control | Stage I–II OPSCC | Complement component C9 | C9 | 0.12 | 0.91 |
| P41222 | 2 | 2 | 11.91 | 0 | 13700.40 | Control | Stage I–II OPSCC | Prostaglandin-H2 D-isomerase | PTGDS | 0.02 | 0.91 |
| P00747;Q02325;Q15195 | 154 | 132 | 1214.37 | 2.25E−11 | 2.00 | Control | Stage I–II OPSCC | Plasminogen | PLG | 0.19 | 0.91 |
| Q9NZP8 | 3 | 2 | 19.85 | 2.79E−07 | 194.65 | Control | Stage I–II OPSCC | Complement C1r subcomponent-like protein | C1RL | 0.02 | 0.92 |
| P80108 | 7 | 7 | 57.61 | 4.83E−11 | 3.28 | Control | Stage I–II OPSCC | Phosphatidylinositol–glycan-specific phospholipase D | GPLD1 | 0.03 | 0.92 |
| P0C0L5 | 394 | 16 | 2558.66 | 2.67E−08 | 6.78 | Control | Stage I–II OPSCC | Complement C4-B | C4B | 0.11 | 0.93 |
| P01031 | 131 | 116 | 977.48 | 3.05E−13 | 2.28 | Control | Stage I–II OPSCC | Complement C5 | C5 | 0.13 | 0.93 |
| Q9Y2H2 | 7 | 5 | 31.34 | 1.11E−16 | 14.32 | Control | Stage I–II OPSCC | Phosphatidylinositide phosphatase SAC2 | INPP5F | 0.08 | 0.93 |
| P09871 | 50 | 42 | 381.14 | 1.18E−13 | 2.02 | Control | Stage I–II OPSCC | Complement C1s subcomponent | C1S | 0.08 | 0.93 |
| Q96KN2 | 18 | 16 | 138.56 | 0 | 3.87 | Control | Stage I–II OPSCC | Beta-Ala-His dipeptidase | CNDP1 | 0.05 | 0.94 |
| P0C0L4 | 393 | 14 | 2503.26 | 9.99E−16 | 49.09 | Control | Stage I–II OPSCC | Complement C4-A | C4A | 0.19 | 0.94 |
| P08294 | 4 | 3 | 29.94 | 0 | 36.98 | Control | Stage I–II OPSCC | Extracellular superoxide dismutase [Cu–Zn] | SD3 | 0.02 | 0.94 |
| Q7LC44 | 3 | 2 | 22.46 | 7.34E−13 | 316.16 | Control | Stage I–II OPSCC | Activity-regulated cytoskeleton-associated protein | ARC | 0.02 | 0.94 |
| O95445 | 12 | 11 | 85.78 | 1.55E−15 | 3.46 | Control | Stage I–II OPSCC | Apolipoprotein M | APM | 0.06 | 0.94 |
| P02649 | 70 | 62 | 492.63 | 3.32E−14 | 3.40 | Control | Stage I–II OPSCC | Apolipoprotein E | APE | 0.16 | 0.94 |
| P27918 | 10 | 8 | 82.75 | 0 | 3.61 | Control | Stage I–II OPSCC | Properdin | CFP | 0.04 | 0.94 |
| P22352 | 23 | 20 | 129.93 | 1.11E−16 | 3.73 | Control | Stage I–II OPSCC | Glutathione peroxidase 3 | GPX3 | 0.07 | 0.95 |
| P07358 | 60 | 51 | 461.28 | 2.22E−16 | 2.42 | Control | Stage I–II OPSCC | Complement component C8 beta chain | C8B | 0.07 | 0.95 |
| P22792 | 28 | 25 | 266.69 | 3.08E−14 | 2.57 | Control | Stage I–II OPSCC | Carboxypeptidase N subunit 2 | CPN2 | 0.09 | 0.95 |
| Q08629 | 4 | 2 | 34.99 | 1.01E−08 | 31.43 | Control | Stage I–II OPSCC | Testican-1 | SPCK1 | 0.03 | 0.95 |
| Q04756 | 8 | 7 | 54.20 | 0 | 228.12 | Control | Stage I–II OPSCC | Hepatocyte growth factor activator | HGFAC | 0.04 | 0.95 |
| P00736 | 54 | 43 | 423.82 | 2.89E−15 | 2.58 | Control | Stage I–II OPSCC | Complement C1r subcomponent | C1R | 0.10 | 0.95 |
| O75636 | 8 | 7 | 88.24 | 0 | 24.29 | Control | Stage I–II OPSCC | Ficolin-3 | FCN3 | 0.07 | 0.95 |
| A0A096LPE2;P35542 | 35 | 15 | 202.65 | 0 | 6.02 | Control | Stage I–II OPSCC | Protein SAA2–SAA4 | SAA2–SAA4 | 0.06 | 0.96 |
| P06681 | 31 | 8 | 254.03 | 6.01E−13 | 4.48 | Control | Stage I–II OPSCC | Complement C2 | C2 | 0.04 | 0.96 |
| P19827 | 126 | 107 | 848.22 | 3.33E−16 | 3.17 | Control | Stage I–II OPSCC | Inter-alpha-trypsin inhibitor heavy chain H1 | ITIH1 | 0.24 | 0.96 |
| P15169 | 10 | 9 | 93.43 | 0 | 9.89 | Control | Stage I–II OPSCC | Carboxypeptidase N catalytic chain | CPN1 | 0.06 | 0.96 |
| Q13790 | 3 | 2 | 27.72 | 1.10E−13 | 22.60 | Control | Stage I–II OPSCC | Apolipoprotein F | APF | 0.02 | 0.96 |
| P07360 | 19 | 15 | 166.71 | 2.64E−14 | 2.87 | Control | Stage I–II OPSCC | Complement component C8 gamma chain | C8G | 0.09 | 0.96 |
| P02747 | 8 | 8 | 60.45 | 6.85E−14 | 4.08 | Control | Stage I–II OPSCC | Complement C1q subcomponent subunit C | C1QC | 0.05 | 0.96 |
| P04070 | 4 | 2 | 23.64 | 1.11E−15 | 4.71 | Control | Stage I–II OPSCC | Vitamin K-dependent protein C | PRC | 0.01 | 0.96 |
| P13645 | 16 | 15 | 115.79 | 0 | 6.25 | Control | Stage I–II OPSCC | Keratin, type-I cytoskeletal 10 | KRT10 | 0.06 | 0.96 |
| P00748 | 3 | 3 | 18.59 | 0 | 679.60 | Control | Stage I–II OPSCC | Coagulation factor XII | F12 | 0.03 | 0.97 |
| P04003 | 82 | 74 | 628.10 | 1.11E−16 | 2.84 | Control | Stage I–II OPSCC | C4b-binding protein alpha chain | C4BPA | 0.18 | 0.97 |
| Q7Z794 | 6 | 3 | 44.34 | 0 | 48569.15 | Control | Stage I–II OPSCC | Keratin, type-II cytoskeletal 1b | KRT77 | 0.05 | 0.97 |
| P07225 | 32 | 27 | 244.61 | 0 | 3.08 | Control | Stage I–II OPSCC | Vitamin K-dependent protein S | PRS1 | 0.09 | 0.97 |
| P06276 | 7 | 6 | 56.00 | 0 | 11.44 | Control | Stage I–II OPSCC | Cholinesterase | BCHE | 0.04 | 0.97 |
| P27482 | 2 | 2 | 11.92 | 1.34E−07 | 25.89 | Control | Stage I–II OPSCC | Calmodulin-like protein 3 | CALML3 | 0.02 | 0.97 |
| P49908 | 9 | 7 | 46.07 | 0 | 41.43 | Control | Stage I–II OPSCC | Selenoprotein P | SELENP | 0.04 | 0.98 |
| O00750 | 11 | 4 | 59.49 | 0 | 4.02 | Control | Stage I–II OPSCC | Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta | PIK3C2B | 0.05 | 0.98 |
| P08571 | 6 | 6 | 51.89 | 0 | 6.40 | Control | Stage I–II OPSCC | Monocyte differentiation antigen CD14 | CD14 | 0.03 | 0.98 |
| Q9UGM5 | 8 | 8 | 54.43 | 1.08E−14 | 13.76 | Control | Stage I–II OPSCC | Fetuin-B | FETUB | 0.03 | 0.98 |
| P35858 | 32 | 29 | 277.24 | 0 | 11.46 | Control | Stage I–II OPSCC | Insulin-like growth factor-binding protein complex acid-labile subunit | IGFALS | 0.11 | 0.98 |
| O75882 | 22 | 20 | 150.98 | 0 | 15.94 | Control | Stage I–II OPSCC | Attractin | ATRN | 0.06 | 0.98 |
| P60709;P63261;P62736;P63267;P68032;P68133 | 6 | 3 | 45.19 | 0 | Infinity | Control | Stage I–II OPSCC | Actin, cytoplasmic 1 | ACTB | 0.05 | 0.99 |
| Q96IY4 | 5 | 5 | 35.72 | 0 | 18.33 | Control | Stage I–II OPSCC | Carboxypeptidase B2 | CPB2 | 0.04 | 0.99 |
| P17936 | 8 | 6 | 58.46 | 0 | 21.95 | Control | Stage I–II OPSCC | Insulin-like growth factor-binding protein 3 | IGFBP3 | 0.03 | 0.99 |
| P06396 | 76 | 3 | 631.02 | 1.11E−16 | 407.24 | Control | Stage I–II OPSCC | Gelsolin | GSN | 0.06 | 0.99 |
| P02746 | 12 | 11 | 106.18 | 0 | 8.16 | Control | Stage I–II OPSCC | Complement C1q subcomponent subunit B | C1QB | 0.07 | 0.99 |
| Q14520 | 19 | 17 | 146.18 | 0 | 7.40 | Control | Stage I–II OPSCC | Hyaluronan-binding protein 2 | HABP2 | 0.07 | 0.99 |
| Q5T6V5 | 5 | 4 | 38.73 | 0 | 64.40 | Control | Stage I–II OPSCC | UPF0553 protein C9orf64 | C9orf64 | 0.09 | 0.99 |