Fig. 1.

Identification and validation of fusion genes in CRC. a Overview of fusion gene filtering process. b Cross-validation with deFuse and FusionMap algorithms prior to PCR validation. GFP algorithm was used as the first detection tool, and then deFuse and FusionMap algorithms were applied to the 25 genes. c RT-PCR validation. Sanger sequencing confirmed 24 out of 25 fusion genes. An arrow denotes a junction point of a fusion gene