Fig. 4.

Biological function test. a Cell proliferation in CRC cells. Each MTT assay was performed three times. Empty vector is pCMV6-myc-DDK, except RNF121-FOLR2, which used pcDNA3.1-V5-His B. *P < 0.05 compared to empty vector, determined by the Student’s t-test; ^#P < 0.05 compared to each acceptor gene expression vector, determined by the Student’s t-test. b Cell migration assay of NAGLU-IKZF3 was performed three times. Harvested cells were seeded in transwell 48 h after transfection. *P < 0.05 compared to empty vector, determined by the Student’s t-test