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. 2018 Jul 16;8:10734. doi: 10.1038/s41598-018-28953-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Schematic diagram of truncated pBnPP2-15-2086 fused GUS constructs and distribution of cis-acting elements. The initiation codon was defined as +1. The different elements were indicated by different color patches. The position of the scissors indicates the location of the truncation of the promoter. The numbers on the left indicated the length of 5′- deletion fragments. (B) GUS histochemical assays in transgenic T3 Arabidopsis seedlings, flowers, mature leaves and siliques carrying pBnPP2-15-2086, pBnPP2-15-1455, pBnPP2-15-1039 and pBnPP2-15-579 constructs. CK−, wild-type; CK+, carrying CaMV 35S promoter constructs. a, b, c and d indicated seedlings, flowers, mature leaves and siliques, respectively. Bar = 0.1 mm. (C) GUS histochemical assays in mature leaves of transgenic T3 Arabidopsis under mechanical wounding and aphid infestation. a indicated leaf from wild-type for control; b, c, d and e indicated leaf from transgenic T3 Arabidopsis lines carrying pBnPP2-15-2086, pBnPP2-15-1455, pBnPP2-15-1039 and pBnPP2-15-579 constructs under mechanical wounding, respectively. f indicated transgenic T3 Arabidopsis lines carrying pBnPP2-15-2086 under aphid infestation. The red marker in Fig. 6Cf was enlarged in the upper right corner. Bar = 0.1 mm.