Fig. 2.

Insulin/Snail1 axis suppresses de novo lipogenesis in hepatocytes. a–c Primary hepatocytes were isolated from Snail1^Δhep and Snail1^flox/flox littermates and stimulated with insulin (50 nM) or PBS for 12 h (a, c) or 4 h (b). a De novo lipogenesis assays (normalized to protein levels; n = 3). b Fasn, Acc1, and Acl mRNA levels (normalized to 36B4 levels; n = 4). c Cell extracts were immunoblotted with the indicated antibodies. d–f Primary hepatocytes were transduced with Snail1 or GFP adenoviral vectors and stimulated with insulin (50 nM) for 12 h (d, f) or 4 h (e). d De novo lipogenesis (normalized to protein levels; n = 3). e Fasn, Acc1, and Acl mRNA levels (normalized to 36B4 levels; n = 4). f Cell extracts were immunoblotted with the indicated antibodies. Data are presented as mean ± SEM. *p < 0.05 by two-tailed unpaired Student’s t test