Fig. 5.

Snail1 directly suppresses liver lipogenic programs in vivo. a, b Snail1^∆hep (n = 6) and Snail1^flox/flox (n = 6) males fed a normal chow diet for 18 weeks. c, d Snail1^flox/flox male mice (8–9 weeks) were fed a HFD for 6 weeks and transduced with AAV-TBG-GFP (n = 6) or AAV-TBG-Cre (n = 6) vectors. Livers were harvested 4 weeks after AAV transduction. e, f C57BL/6 male mice (8–9 weeks) were fed a HFD for 7 weeks and transduced with Snail1 (n = 5) or GFP (n = 5) adenoviral vectors. e Liver Fasn, Acc1, and Acl expression was assessed by qPCR (normalized to 36B4 expression). f Liver extracts were immunoblotted with the indicated antibodies. Fasn, Acc1, and Acl levels were quantified and normalized to α-tubulin levels. Data are presented as mean ± SEM. *p < 0.05, two-tailed unpaired Student’s t test