Fig. 6.

Snail1 epigenetically represses the Fasn promoter. a Male mice were transduced with Snail1 adenoviral vectors for 3 weeks. Binding of Snail1 to the Fasn promoter was assessed in the liver by ChIP. b Fasn luciferase activity (normalized to β-gal internal controls). c Male mice were transduced with Snail1 adenoviral vectors for 3 weeks. Liver extracts were immunoprecipitated with antibody against Snail1 and immunoblotted with antibodies against HDAC1 or HDAC2. d C57BL/6 male mice (8–9 weeks) were fed a HFD for 7 weeks and then transduced with Snail1 (n = 3) or GFP (n = 3) adenoviral vectors. Livers were harvested 3 weeks after transduction. The levels of liver H3K9ac and H3K27ac on the Fasn, NCH, or Actb promoter were measured by ChIP-qPCR and normalized to inputs. e Snail1^∆hep (n = 3) and Snail1^flox/flox (n = 3) males were fed a HFD for 10 weeks. The levels of liver H3K9ac or H3K27ac on the Fasn, NCH, or Actb promoter were measured by ChIP. f, g Primary hepatocytes were transduced with Snail1, ΔN20, or GFP adenoviral vectors and stimulated with or without insulin (50 nM) for 12 h. f Lipogenesis. g H3K27ac levels on the Fasn promoter. Data are presented as mean ± SEM. *p < 0.05, two-tailed unpaired Student’s t test